The total number of colonies refers to the total number of colonies contained in 1mL (g) of the test sample after the food inspection is homogenized and cultured under certain conditions (medium composition, temperature, time, pH, aerobic properties, etc.), generally nutrition The agar was incubated at 36 ° C for 48h, and the total number of colonies of aerobic and facultative anaerobic bacteria was obtained. The total number of colonies is mainly used to determine the index of food contamination by bacteria, used to check the cleanliness of food raw materials, whether food is handled properly, freshness and quality. The principle is the dilution method. The colonies formed on the nutrient agar plate may be grown by one bacterium, or may be grown by more than one bacterium, so the final report is the number of colony formation units.

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